Journal: Animals : an Open Access Journal from MDPI

Article Title: Liver Histopathological and Immunohistochemical Evaluation from Fasciola hepatica Experimentally Infected and Reinfected Sheep

doi: 10.3390/ani14121833

Figure Lengend Snippet: Photomicrograph of CD3+ T cells (red-brown color) in a granuloma. CD3+ cells (arrows) are mainly located in the periphery of a granuloma in 16 dpi reinfected sheep. Avidin–biotin–peroxidase complex (ABC) method.

Article Snippet: Biotinylated immunoglobulin serum (Dako-Agilent, Santa Clara, CA, USA) was diluted 1:200 and applied to the slides incubated with the primary polyclonal antibodies (goat anti-rat for anti-Foxp3 and goat anti-rabbit for anti-iNOS and anti-CD3), whereas biotinylated goat anti-mouse immunoglobulin serum (Dako-Agilent, Santa Clara, CA, USA), diluted 1:50, was used for the slides incubated with the primary monoclonal antibody anti-CD163).

Techniques: Avidin-Biotin Assay

Journal: Animals : an Open Access Journal from MDPI

Article Title: Liver Histopathological and Immunohistochemical Evaluation from Fasciola hepatica Experimentally Infected and Reinfected Sheep

doi: 10.3390/ani14121833

Figure Lengend Snippet: Photomicrograph of Foxp3+ cells (red-brown color) in the uninfected control ( A ) and a reinfected group ( B ). ( A ). Absence of Foxp3+ cells (arrows) in the portal infiltrate of an uninfected control sheep. ( B ). Foxp3+ cells (arrows) in a portal space in 4 dpi reinfected sheep. Avidin–biotin–peroxidase complex (ABC) method.

Article Snippet: Biotinylated immunoglobulin serum (Dako-Agilent, Santa Clara, CA, USA) was diluted 1:200 and applied to the slides incubated with the primary polyclonal antibodies (goat anti-rat for anti-Foxp3 and goat anti-rabbit for anti-iNOS and anti-CD3), whereas biotinylated goat anti-mouse immunoglobulin serum (Dako-Agilent, Santa Clara, CA, USA), diluted 1:50, was used for the slides incubated with the primary monoclonal antibody anti-CD163).

Techniques: Avidin-Biotin Assay

Journal: Animals : an Open Access Journal from MDPI

Article Title: Liver Histopathological and Immunohistochemical Evaluation from Fasciola hepatica Experimentally Infected and Reinfected Sheep

doi: 10.3390/ani14121833

Figure Lengend Snippet: Photomicrograph of CD163+ cells (red-brown color) at 100 dpi in primoinfected group ( A ) and uninfected control group ( B ). ( A ). Numerous CD163+ cells (arrows) are observed in the inflammatory infiltrate associated with an enlarged bile duct containing an F. hepatica parasite in 100 dpi primoinfected sheep. ( B ). Absence of CD163+ cells in portal spaces (arrows) and hepatic sinusoids of an uninfected control sheep. Avidin–biotin–peroxidase complex (ABC) method.

Article Snippet: Biotinylated immunoglobulin serum (Dako-Agilent, Santa Clara, CA, USA) was diluted 1:200 and applied to the slides incubated with the primary polyclonal antibodies (goat anti-rat for anti-Foxp3 and goat anti-rabbit for anti-iNOS and anti-CD3), whereas biotinylated goat anti-mouse immunoglobulin serum (Dako-Agilent, Santa Clara, CA, USA), diluted 1:50, was used for the slides incubated with the primary monoclonal antibody anti-CD163).

Techniques: Avidin-Biotin Assay